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浅析阳春砂花药发育过程的组织化学研究

Histochemical Study on Anther Development of Ambrosia Solanum L

现代分析化学研究 / 2022,4(3)：35-40 / 2022-11-09 192 254

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• 作者： 刘慧     
• 单位：

  湖北中医药大学，武汉
  

• 关键词： 阳春砂；花药发育；淀粉；脂滴
• Amomum solanum; Anther development; Starch; Lipid drops
• 摘要： 用环氧树脂包埋的半薄切片经PAS反应和苏丹黑染色，研究阳春砂花药发育中的多糖和脂类物质分布特征。结果发现小孢子母细胞和四分体小孢子中积累了 一些脂滴，但没有淀粉。阳春砂小孢子母细胞和四分体没有胼胝质壁。晚期小孢子中除了仍有很多脂滴外，细胞核周围开始出现淀粉粒；成熟花粉粒贮存丰 富的淀粉粒和脂滴，且花粉壁由多糖物质构成。阳春砂花药壁结构比较特殊：花药壁由10余层细胞组成；最内层的绒毡层细胞在小孢子时期开始解体，细胞 质转变为脂滴，供花粉吸收。开花时，花药壁由表皮和几层薄壁细胞以及径向壁纤维加厚的变形细胞组成。
• The distribution characteristics of polysaccharides and lipids in anther development of Ambrosium solanum were studied by PAS reaction and Sudan black staining in semi-thin sections embedded in epoxy resin. Some lipid droplets but no starch were found in microspore mother cells and tetrad microspores. The microspore blasts and tetrads of A. solanum have no callose wall. In addition to many lipid droplets, starch grains began to appear around the nucleus in the late microspores. Mature pollen grains store abundant starch grains and lipid droplets, and the pollen wall is composed of polysaccharides. The anther wall structure of A. solanum is special: the anther wall is composed of more than 10 layers of cells. The innermost tapetum cells begin to disintegrate at the microspore stage, and the cytoplasm turns into lipid droplets for pollen absorption. At flowering, the anther wall is composed of epidermis and several layers of parenchyma cells and deformed cells with thickened radial wall fibers.
• DOI： https://doi.org/10.35534/macr.0403004c
• 引用： 刘慧．浅析阳春砂花药发育过程的组织化学研究［J］．现代分析化学研究，2022，4（3）：35-40．