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基于HPLC法的双氯芬酸钠测定

Determination of diclofenac sodium by HPLC method

现代分析化学研究 / 2019,1(1)：13-17 / 2019-12-27 497 543

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• 作者： 龚文涛      陈晨*     
• 单位：

  沈阳师范大学，沈阳

• 关键词： 高效液相色谱法；双氯芬酸钠栓；双氯芬酸钠；含量测定
• High performance liquid chromatography; Diclofenac sodium suppository; Diclofenac sodium; Content determination
• 摘要： 目的：采用高效液相色谱（HPLC）法测定双氯芬酸钠栓中双氯芬酸钠 的含量。方法：色谱柱为Hypersil GOLDC1s 柱（250 mm×4.6 mm，5um），以0.1 mol/L 冰醋酸溶液- 乙腊（50：50）为流动相，检测波长为282 nm。结果：双 氯芬酸钠的平均回收率为99.31%，RSD=0.83%（n=5），质量浓度在63.2~147.2 ug/mL 范围内与峰面积线性关系良好。结论HPLC 法简便、快捷、准确。
• Objective: to determine the content of diclofenac sodium in diclofenac sodium suppository by high performance liquid chromatography (HPLC). Methods: the chromatographic column was Hypersil GOLDC1s (250 mm×4.6 mm, 5um), the mobile phase was 0.1 mol/L glacial acetic acid solution - ethylene (50:50), and the detection wavelength was 282 nm. Results: the average recovery rate of diclofenac sodium was 99.31%, RSD=0.83% (n=5), and the mass concentration had a good linear relationship with the peak area in the range of 63.2~ 147.2ug /mL. Conclusion HPLC method is simple, fast and accurate.
• DOI： https://doi.org/10.35534/macr.0101003c
• 引用： 龚文涛，陈晨．基于 HPLC 法的双氯芬酸钠测定［J］．现代分析化学研究，2019，1（1）：13-17．