Abstract:
Objective: to determine the content of diclofenac sodium in diclofenac
sodium suppository by high performance liquid chromatography (HPLC). Methods:
the chromatographic column was Hypersil GOLDC1s (250 mm×4.6 mm, 5um), the
mobile phase was 0.1 mol/L glacial acetic acid solution - ethylene (50:50), and the
detection wavelength was 282 nm. Results: the average recovery rate of diclofenac
sodium was 99.31%, RSD=0.83% (n=5), and the mass concentration had a good linear
relationship with the peak area in the range of 63.2~ 147.2ug /mL. Conclusion HPLC
method is simple, fast and accurate.
目的:采用高效液相色谱(HPLC)法测定双氯芬酸钠栓中双氯芬酸钠
的含量。方法:色谱柱为Hypersil GOLDC1s 柱(250 mm×4.6 mm,5um),以0.1
mol/L 冰醋酸溶液- 乙腊(50:50)为流动相,检测波长为282 nm。结果:双
氯芬酸钠的平均回收率为99.31%,RSD=0.83%(n=5),质量浓度在63.2~147.2
ug/mL 范围内与峰面积线性关系良好。结论HPLC 法简便、快捷、准确。