Abstract:
Objective: The neuroprotective and anti-inflammatory effects of Shexiang Baoxin Pill (SBP) were investigated after different extraction methods. Methods: Nerve growth factor (NGF) was used to induce the differentiation of cultured PC12 cells to establish the neurite outgrowth model. In addition, lipopolysaccharide (LPS)-induced inflammation in RAW264.7 cell was used as the inflammatory model. SBP extracts were prepared by different extraction methods, and the optimal dosages were determined by MTT assay. By the morphological analysis, the changes of neurite length were detected, and the Aβ1-42 aggregation-induced cytotoxicity of PC12 was determined by MTT. The mRNA level of inflammatory cytokine was examined by RT-PCR. Results: SBP extracts could promote neurite growth and reverse the Aβ1-42 aggregation-induced neurotoxic effect in PC12 cells (p<0.05). SBP extracts significantly inhibited the expressions of tumor necrosis factor (TNF-α) and interleukin-6 (IL-6) in RAW264.7 cells (p<0.05), except the extract of SBP by using simulated gastric fluid. Conclusion: SBP has neuroprotective potential and can attenuate the LPS-induced inflammatory responses.
目的:探究不同前处理的麝香保心丸(Shexiang Baoxin Pill,SBP)的神经保护作用及抗炎反应。方法:本研究利用神经生长因子诱导PC12细胞分化建立轴突生长模型,利用脂多糖诱导RAW264.7细胞建立炎症模型。用不同前处理方式得到SBP药液,通过四甲基偶氮唑盐(MTT)法确定最佳给药剂量。通过形态学分析得到PC12轴突长度的变化;MTT法测定Aβ1-42聚集对PC12的细胞毒性;荧光定量PCR法测定SBP对炎症因子表达的影响。结果:SBP对神经轴突生长具有促进作用,且可显著地逆转由Aβ1-42聚集产生的神经毒性作用(p<0.05);除人工胃液处理的SBP外,其余SBP均可显著抑制肿瘤坏死因子(TNF-α)和白介素-6(IL-6)表达水平(p<0.05)。结论:SBP具有神经保护潜力,且可以抑制由LPS诱导产生的炎症反应。