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反相层析技术在多肽纯化中的优化策略及环境友好性探讨

Optimization Strategies and Environmental Friendliness of Reversed-phase Chromatography in Peptide Purification

Environment and Resource / 2026,8(1): 61-65 / 2026-02-28 10 4

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• Authors: 周光旭 方宝 易旭东 刘敏
• Information:

  太极集团重庆涪陵制药厂有限公司，重庆

• Keywords:
  Reversed-phase high-performance liquid chromatography; Peptide purification; Green solvents; Waste liquid management; Optimization strategies
  反相高效液相色谱; 多肽纯化; 绿色溶剂; 废液管理; 优化策略
• Abstract: Reversed-phase high-performance liquid chromatography (RP-HPLC) is an effective method for separating complex peptide mixtures and currently the most widely used technique for peptide purification. Traditional RP-HPLC processes face challenges such as high solvent consumption and environmental issues related to waste liquid disposal. Therefore, optimizing RP-HPLC processes to improve purification efficiency and reduce environmental impact has become a focal point of current research. This article summarizes the application of RP-HPLC in peptide purification and explores several optimization strategies, including fine-tuning chromatographic conditions, the use of novel stationary phase materials, and the substitution with green solvents. Environmentally friendly alternatives such as wateralcohol systems, ionic liquids, and deep eutectic solvents are proposed as green solvent options. By integrating ultrahigh- performance liquid chromatography (UHPLC) with other technologies, such as organic solvent nanofiltration (OSN), purification efficiency can be further enhanced while reducing solvent consumption. Finally, the future development directions of RP-HPLC technology are discussed, highlighting that the combination of intelligent processes and green technologies has the potential to promote more sustainable peptide drug production. 反相高效液相色谱（RP-HPLC）可对复杂的多肽混合物进行有效分离，是现阶段应用最为普遍的多肽纯化方法。传统RP-HPLC工艺存在溶剂消耗高、废液处理环保相关问题。如何优化RP-HPLC工艺，进而提高纯化效率、减轻对环境的负担，成为当下研究的焦点。本文归纳了RP-HPLC在多肽纯化中的应用，接着探讨了几种优化途径，涵盖对色谱条件的精细调控、新型固定相材料的应用以及绿色溶剂的替代措施，水—醇体系、离子液体和低共熔溶剂等绿色溶剂被提出作为环境友好型替代选项。采用超高效液相色谱（UHPLC）和其他集成技术相配合，如耐溶剂纳滤（OSN）这种，有望进一步提高纯化效率，同时减少溶剂消耗，最后对RP-HPLC技术未来的发展方向予以展望，提出采用智能化工艺与绿色化技术相结合，有潜力推动多肽药物生产向更可持续的方向发展。
• DOI: https://doi.org/10.35534/er.0801007 (registering DOI)
• Cite: 周光旭，方宝，易旭东，等．反相层析技术在多肽纯化中的优化策略及环境友好性探讨［J］．环境与资源，2026，8（1）：61-65.