Keywords:
Infantile paracetamol amine granules; Acetaminophen: caffeine: high phase liquid chromatography: determination of content
小儿氨酚烷胺颗粒;对乙酰氨基酚:咖啡因:高相液相色谱法:含量 测定
Abstract:
Objective: to establish a high performance liquid chromatography
(HPLC) method for the determination of acetaminophen and caffeine in infantile
aminophenolamine granules. Methods: using octadecylsilane bonded silica gel as
filler, 0.025mol/L phosphoric acid-acetonitrile (85:15), whose pH was adjusted to 3.0
by triethylamine, was used as mobile phase, the detection wavelength was 271mm,
and the injection volume was 20μL. Results: the linear ranges of paracetamopion and
caffeine were 120~2000 g/mL (r=0.9999) and 2.4~200 g/mL (r=0.9999), respectively.
The mean recovery rate of acetaminophen was 100.33%, RSD=0.56% (n=5). The
average recovery of caffeine was 102.59%, RSD=l.75% (n=5). Conclusion: the method
is simple, fast, accurate, stable and reproducible.
目的:建立测定小儿氨酚烷胺颗粒中对乙酰氨基酚和咖啡因含量的高
效液相色谱(HPLC)法。方法:用十八烷基硅烷键合硅胶为填充剂,以用三
乙胺调pH 至3.0 的0.025mol/L 磷酸溶液- 乙腈(85:15)为流动相,检测波
长为271mm,进样量为20μL。结果:对乙酰氨基盼和咖啡因的线性范围分别
为120 ~ 2000μg/mL(r=0.9999)和2.4 ~ 200μg/mL(r=0.9999)。对乙酰氨
基酚的平均回收率为100.33%,RSD=0.56%(n=5);咖啡因的平均回收率为
102.59%,RSD=l.75%(n=5)。结论:该法简单、快速、准确、稳定、重现性好。