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高效液相色谱测定饲料中三聚氰胺含量的方法

Determination of melamine in feed by HPLC

Modern Analytical Chemistry Research / 2020,2(1): 9-15 / 2020-04-29 2363 4062

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• Authors: 邓勤勤     
• Information:

  南昌大学，南昌

• Keywords: Melamine; High performance liquid chromatography; Feed 三聚氰胺；高效液相色谱；饲料
• Abstract: A high per formance liquid chromatographic method was established for the determination of melamine in feed. The samples were extracted with trichloroacetic acid solution. After centrifuged, the supernatants were cleaned by mixed cation exchange SPE column. The eluant were dried, then redissolved by initial mobile phase. Separation was performed by using Elite MSP column (ID 4.6 mm×150 mm, 5 m), the retention time of melamine was 14 min. The limit of detection (LOD) of the method was 0.046 mg/kg and the limit of quantification (LOQ) was 0.153 mg/kg. The method was sensitive and reliable and more propitious to be used for the determination of melamine in feed. 建立了高效液相色谱（HPLC）测定饲料中三聚氰胺含量的方法。样品 中三聚氰胺用三氯乙酸溶液提取，提取离心后经混合型阳离子交换固相萃取柱净 化，洗脱物吹干后用流动相溶解，用高效液相色谱仪进行测定。使用 Elite MSP 5 m（ID 4.6 mm×150 mm）色谱柱，三聚氰胺保留时间为 14 min，方法验证 结果表明该方法具有良好的稳定性和重现性，该方法的三聚氰胺方法检出限 为 0.046 mg/kg，方法定量限为 0.153 mg/kg，远低于农业部标准 NY/T 1372- 2007 的检出限 2.0 mg/kg, 为饲料样品的安全性评价提供了稳定、高灵敏分析 方法。
• DOI: https://doi.org/10.35534/macr.0201002c
• Cite: 邓勤勤．高效液相色谱测定饲料中三聚氰胺含量的方法［J］．现代分析化学研究，2020，2（1）：9-15．