Abstract:
The anesthetic effect of different concentrations of MS-222 on Brachymystax lenok Pallas was observed. The results showed that the anesthetic effect of juvenile fish was a gradual process, which can be divided into four groups: non-anaesthesia, light anesthesia, moderate anesthesia and deep anesthesia. There were differences in the time of entering different anaesthesia degree at different concentration of MS-222. In the process of 900 s treated with MS-222, 100% of juvenile fish showed light anaesthesia under the concentration of 30 mg/l. At 301.5 s, juvenile fish all entered mild anesthesia during the concentration of 35 mg/l, 567 s showed moderate anesthesia. At 57.5 s, juvenile fish all entered mild anesthesia during the concentration of 40 mg/l, 638.5 s showed deep anesthesia. During the experiment, 100% of 45 mg/l ~ 55 mg/l showed deep anesthesia, and the duration of all anesthesia was shortened gradually. When the concentration exceeded 45 mg/l, and the anesthesia time exceeded 12 hours, the juvenile fish died. Therefore, attention should be paid to the concentration and time of the anesthetic MS-222.
本实验研究了不同浓度MS-222对细鳞鱼幼鱼的麻醉效果。结果表明:细鳞鱼幼鱼的麻醉过程可分为不被麻醉、轻度麻醉、中度麻醉和深度麻醉;在不同浓度MS-222条件下,细鳞鱼幼鱼进入不同麻醉程度的时间有差异;在30 mg/l浓度下,MS-222处理 900 s,细鳞鱼幼鱼100%呈现轻度麻醉;在35 mg/l浓度下,301.5 s幼鱼全部进入轻度麻醉,567 s呈现中度麻醉;在40 mg/l浓度下,57.5 s幼鱼全部进入轻度麻醉,638.5 s呈现深度麻醉;在45 mg/l,120 s幼鱼直接进入中度麻醉,528.5 s呈现深度麻醉;在45 mg/l~55 mg/l浓度范围,幼鱼100%呈现深度麻醉的情况,且麻醉时间逐渐缩短。浓度超过45 mg/l,麻醉时间超过12 h,细鳞鱼幼鱼出现了死亡,因此使用麻醉剂MS-222需要注意浓度和使用时间。